A simple cell-free enzymatic platform for large-scale DNA manufacturing

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Linea™IVT — Better mRNA…Faster

A Combination of 2 Next Generation Technologies

Improved mRNA Graphic

LineaIVT is the combination of LRx’s enzymatically produced LinearDNA IVT templates and its proprietary Linea RNA polymerase. The unique combination redefined mRNA production, resulting in:

A simplified mRNA workflow

Conventional IVT mRNA Production

mRNA product Encapsulate and sterile filtration Purification step 3 Purification step 2 Purification step 1 In vitro transcription pDNA linearization and purification pDNA IVT Template Manufacture

Linea IVT mRNA Production

mRNA product Encapsulate and sterile filtration Purification step 2 Purification step 1 In vitro transcription LinearDNA IVT Template Manufacture

With significantly Reduced or Eliminated dsRNA Impurities

reduced impurities graphic

Without Sacrificing Yield

LineaIVT Yield Graphic

linDNA offers key advantages compared to pDNA:

Attribute
LinearDNA
Plasmid DNA
Risk of Antibiotic Resistance Gene Transfer
Endotoxin Risk
Cellular Purification Necessary
Know/Fixed Yield
Unwanted DNA in Final Product
Manufacturing Timeframe
Days
Weeks/Months
DNA Construct Optimization via Primer Modification
Able to Produce Long Homogenous Poly-A Tails (necessary for mRNA templates)
Percentage of Produced DNA Comprised of Target Therapeutic DNA Sequence
100%
≈ 40%

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